Practical Bioinformatics I
Biological Data Manipulation
What you will learn
Sanger sequencing method and working with trace files
Common High-throughput sequencing platforms, methods, and applications
Common sequence, feature, alignment, and variation files
Extract, convert, split, merge, and/or remove sequences, features, alignments, and/or variations
Using more than 40 (currently 43) bioinformatics programs in addition to explaining their parameters
Using more than 20 (currently 21) bioinformatics programs without explaining their parameters
Different ways of using biological data in different researches (more than 30 papers are available)
Why take this course?
🎉 Practical Bioinformatics I: Mastering Biological Data Manipulation with Ahmed Karam, M.Sc. 🧬👩💻
Headline: Dive into the world of bioinformatics and master the art of manipulating biological data with our comprehensive online course! Led by the experienced instructor Ahmed Karam, M.Sc., this course is designed to equip you with the essential tools and techniques needed to accurately interpret and handle biological files, their components, and their outputs.
Course Description:
Biological data manipulation is a critical skill in bioinformatics, and understanding how to work with nucleic acids is fundamental. In this course, you'll learn to use popular, free, and accessible software programs to effectively manage biological data. We kick off with an exploration of the Sanger Sequencing method—a technique that has laid the groundwork for modern sequencing technology—and then delve into the cutting-edge world of high-throughput sequencing.
Key Takeaways:
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Sanger Sequencing Fundamentals:
- Learn about the Sanger Sequencing method, its file formats, common issues, and their causes.
- Engage in practical applications to open and manipulate Sanger Sequencing files using three different programs.
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High-throughput Sequencing Techniques:
- Get to grips with the various high-throughput sequencing methods like Illumina, Ion Torrent, Pacific Biosciences, and Oxford Nanopore.
- Discover the wide array of applications for high-throughput sequencing in different scientific fields.
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Working with Bioinformatics Files:
- Explore the most commonly used bioinformatics files, including FASTQ, FASTA, Genbank, GTF/GFF3, BED, SAM/BAM, BCF/VCF, and understand their roles in biological research.
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Practical Applications:
- From Sequence Manipulation Suite 2 to EMBOSS, learn how to use various online and software-installed programs to manipulate the files discussed.
- Get hands-on experience with essential Galaxy platform packages like Seqtk, Bedtools, Samtools, and Bcftools.
Course Structure:
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Part 1: Sanger Sequencing Methodologies 🧬✅
- Understanding the method and its outputs.
- Practical exercises to handle Sanger Sequencing files.
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Part 2: High-throughput Sequencing Techniques 🔬🚀
- Overview of high-throughput sequencing methods.
- Applications in diverse scientific fields.
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Part 3: Bioinformatics Files Explained 📁💧
- A comprehensive look at the most common bioinformatics file types and formats.
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Parts 4 to End: Practical Bioinformatics Applications 🛠️👨💻
- Hands-on practice using various programs to manipulate biological data files.
- A blend of detailed explanations for fundamental programs and targeted functions for additional tools.
Additional Features:
- Interactive Quizzes: While we're working on completing the quizzes, rest assured that they will be a crucial part of testing your knowledge and understanding of the course material.
Join us in this practical journey into bioinformatics! With Ahmed Karam's expert guidance, you'll gain the skills needed to navigate the vast landscape of biological data with confidence. Enroll now and take your first steps towards becoming a bioinformatics specialist! 🌟✨
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