Detailed polymerase chain reaction concepts

PCR

3.94 (8 reviews)
Udemy
platform
English
language
Science
category
instructor
Detailed polymerase chain reaction concepts
20
students
2 hours
content
Sep 2021
last update
$27.99
regular price

What you will learn

what is PCR?

PCR types?

Steps of PCR

Applications

Why take this course?

🚀 Unlock the Secrets of Molecular Biology: Mastering PCR!

🔥 Discover the Power of Polymerase Chain Reaction (PCR) 🧬

Introduction to PCR:

  • What is PCR and why is it a game-changer in molecular biology?
  • The brain behind PCR: Meet the Nobel laureates who revolutionized DNA analysis.
  • A step-by-step guide on how PCR works, breaking down the science into digestible chunks.
  • Explore the key chemical components essential for PCR to thrive.
  • Visualize the process with a comprehensive PCR diagram that demystifies each stage.

Understanding PCR's Versatility: 🔍 Types of PCR and Their Applications:

  • Learn about Quantitative PCR (qPCR) and its role in precise measurement of DNA levels.
  • Delve into the world of Real Time PCR, where results are obtained while the reaction is still underway!
  • Uncover the power of Reverse Transcription PCR for RNA detection and analysis.
  • Discover specialized PCR techniques like Multiplex, Nested, High-Fidelity, Fast, Heart Start, GC Rich, Long Range, and Arbitrarily Primed (AP) PCR, and their unique advantages.

PCR in Action: 🌐 Diverse Applications of PCR:

  • Explore PCR's role in clinical diagnosis, prenatal testing, retro-viral and bacterial infection detection, cancer diagnosis, sex determination in embryos, and forensic medicine.
  • Understand the standard ingredients required for a successful PCR reaction: your DNA segment of interest, specific primers, heat-resistant DNA polymerase enzyme, and the four types of nucleotides, complemented by the right salts to create an optimal environment for amplification.

Advanced PCR Techniques:

  • Get an in-depth look at Quantitative PCR (qPCR), also known as Real-Time PCR, and its significance in accurately measuring DNA concentrations.
  • Real-Time PCR: The cutting-edge technique that allows for the detection of products throughout the amplification process, thanks to fluorescent markers.
  • Discover how Reverse Transcription PCR is a vital tool for measuring the amount of a specific RNA in a sample.
  • Learn about direct detection of microorganisms and the identification of antimicrobial resistance patterns.
  • Explore genetic fingerprinting, paternity testing, mutation detection for genetic diseases, and so much more!

Why Enroll in This Course?

  • Gain a comprehensive understanding of PCR technology, its principles, and applications.
  • Access to maximum details in engaging course videos that will bring the science to life.
  • Whether you're a budding scientist, a seasoned researcher, or a curious enthusiast, this course is designed to equip you with the knowledge to leverage PCR for groundbreaking discoveries.

🚀 Embark on your journey to becoming a PCR expert today! 🧫✨

Screenshots

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Related Topics

4306537
udemy ID
18/09/2021
course created date
01/10/2021
course indexed date
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