Detailed polymerase chain reaction concepts
PCR
3.94 (8 reviews)

20
students
2 hours
content
Sep 2021
last update
$27.99
regular price
What you will learn
what is PCR?
PCR types?
Steps of PCR
Applications
Why take this course?
🚀 Unlock the Secrets of Molecular Biology: Mastering PCR!
🔥 Discover the Power of Polymerase Chain Reaction (PCR) 🧬
Introduction to PCR:
- What is PCR and why is it a game-changer in molecular biology?
- The brain behind PCR: Meet the Nobel laureates who revolutionized DNA analysis.
- A step-by-step guide on how PCR works, breaking down the science into digestible chunks.
- Explore the key chemical components essential for PCR to thrive.
- Visualize the process with a comprehensive PCR diagram that demystifies each stage.
Understanding PCR's Versatility: 🔍 Types of PCR and Their Applications:
- Learn about Quantitative PCR (qPCR) and its role in precise measurement of DNA levels.
- Delve into the world of Real Time PCR, where results are obtained while the reaction is still underway!
- Uncover the power of Reverse Transcription PCR for RNA detection and analysis.
- Discover specialized PCR techniques like Multiplex, Nested, High-Fidelity, Fast, Heart Start, GC Rich, Long Range, and Arbitrarily Primed (AP) PCR, and their unique advantages.
PCR in Action: 🌐 Diverse Applications of PCR:
- Explore PCR's role in clinical diagnosis, prenatal testing, retro-viral and bacterial infection detection, cancer diagnosis, sex determination in embryos, and forensic medicine.
- Understand the standard ingredients required for a successful PCR reaction: your DNA segment of interest, specific primers, heat-resistant DNA polymerase enzyme, and the four types of nucleotides, complemented by the right salts to create an optimal environment for amplification.
Advanced PCR Techniques:
- Get an in-depth look at Quantitative PCR (qPCR), also known as Real-Time PCR, and its significance in accurately measuring DNA concentrations.
- Real-Time PCR: The cutting-edge technique that allows for the detection of products throughout the amplification process, thanks to fluorescent markers.
- Discover how Reverse Transcription PCR is a vital tool for measuring the amount of a specific RNA in a sample.
- Learn about direct detection of microorganisms and the identification of antimicrobial resistance patterns.
- Explore genetic fingerprinting, paternity testing, mutation detection for genetic diseases, and so much more!
Why Enroll in This Course?
- Gain a comprehensive understanding of PCR technology, its principles, and applications.
- Access to maximum details in engaging course videos that will bring the science to life.
- Whether you're a budding scientist, a seasoned researcher, or a curious enthusiast, this course is designed to equip you with the knowledge to leverage PCR for groundbreaking discoveries.
🚀 Embark on your journey to becoming a PCR expert today! 🧫✨
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4306537
udemy ID
18/09/2021
course created date
01/10/2021
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