Next Generation Sequencing (NGS)
Learn what is next generation sequencing (NGS) and how it works
What you will learn
Introduction and Historical Background of Next Generation Sequencing
What is Next Generation Sequencing (NGS)?
Key Principle behind NGS
Difference between Sanger sequencing and NGS
Sample preparation (Pre-processing) for NGS
Library Preparation for NGS
PCR Amplification for NGS including Emulsion PCR and Bridge PCR
454 Pyrosequencing approcah
Sequencing by ligation (SOLiD) approach
Ion torrent semiconductor sequencing approach
Reversible terminator sequencing (illumina) approach
Sequencing read types including single end and paired end reads
Data Analysis (Post-processing) for NGS
key considerations when selecting sequencing strategy
Strengths and Limitations of Next Generation Sequencing
Applications of Next Generation Sequencing
Third and Fourth Generation Technologies
Why take this course?
🎉 Unlock the Secrets of DNA with Next Generation Sequencing (NGS)!
🚀 Course Title: Next Generation Sequencing (NGS)
👩🏫 Instructor: Anum Ahmad, PhD
Course Headline: Master the Art of Genomic Analysis with NGS 🧬🔬
Welcome to the World of Next Generation Sequencing (NGS)! Over the past five decades, the field of genomics has been revolutionized by advancements in DNA sequencing technology. The completion of the Human Genome Project marked a turning point, propelling us into a new era of biological discovery and understanding. Today, NGS stands at the forefront of this scientific renaissance, offering unparalleled insights into the genetic blueprints that govern life itself.
Course Description:
NGS technology is a game-changer in the realm of genomic research, transforming the way we analyze DNA and RNA sequences. Unlike the traditional Sanger sequencing method, NGS technologies can process millions of reactions simultaneously, delivering high-throughput sequencing with remarkable speed and efficiency. This course will guide you through the intricacies of NGS, from sample preparation to data analysis, providing a comprehensive understanding of this cutting-edge technology.
What You'll Learn:
🔍 Understanding the Basics: Dive into the principles of NGS and its significance in modern biology.
🧪 Sample Preparation: Explore the process of DNA fragmentation, end repairing, and adaptor ligation that paves the way for library preparation.
⚗️ PCR Amplification Techniques: Learn about emulsion PCR and bridge PCR, two critical methods for amplifying your DNA template.
🖥️ NGS Platforms: Discover the various NGS platforms available, including 454 Pyrosequencing, SOLiD, Ion Torrent Semiconductor Sequencing, and Reversible Terminator Sequencing (Illumina).
🔋 Data Analysis: Gain insights into how to handle the vast amounts of data generated by NGS.
🧩 Applications of NGS: Explore the myriad applications of NGS in fields such as outbreak management, personalized medicine, cancer research, and more.
Key Steps of NGS:
- Sample Preparation (Pre-processing): Tailored to your target DNA or RNA, this step sets the stage for accurate sequencing.
- Library Preparation: Involves DNA fragmentation, end repairing, and adaptor ligation to create a library suitable for sequencing.
- Sequencing: The core process where the order of nucleotides is determined using high-throughput technology.
- Data Analysis (Post-processing): Interpreting the raw data to gain meaningful insights into genomic sequences.
NGS Read Types:
- Single End Reads: A single sequence from one end of a DNA fragment.
- Paired End Reads: Both ends of a DNA fragment are sequenced, providing more context and enabling precise location information in the genome.
NGS has opened up new horizons in genomics, allowing researchers to tackle global challenges and advance personalized medicine. With its applications spanning from outbreak management to the detection of viruses and the surveillance of antimicrobial resistance, NGS is a cornerstone technology in modern research.
Who Should Take This Course?
This course is an invaluable resource for students and researchers in fields such as molecular biology, forensic science, medical laboratory technology, biotechnology, and genetics. Whether you're a beginner or looking to deepen your understanding of NGS, this course will equip you with the knowledge to harness the power of genomic analysis.
🎓 Embark on your learning journey today and become proficient in the world of genomics with Next Generation Sequencing! 🌟
Enroll now and unlock the potential of NGS to propel your research forward!