Next Generation Sequencing (NGS)

Learn what is next generation sequencing (NGS) and how it works

4.20 (115 reviews)
Udemy
platform
English
language
Science
category
instructor
Next Generation Sequencing (NGS)
364
students
1 hour
content
Jan 2022
last update
$44.99
regular price

What you will learn

Introduction and Historical Background of Next Generation Sequencing

What is Next Generation Sequencing (NGS)?

Key Principle behind NGS

Difference between Sanger sequencing and NGS

Sample preparation (Pre-processing) for NGS

Library Preparation for NGS

PCR Amplification for NGS including Emulsion PCR and Bridge PCR

454 Pyrosequencing approcah

Sequencing by ligation (SOLiD) approach

Ion torrent semiconductor sequencing approach

Reversible terminator sequencing (illumina) approach

Sequencing read types including single end and paired end reads

Data Analysis (Post-processing) for NGS

key considerations when selecting sequencing strategy

Strengths and Limitations of Next Generation Sequencing

Applications of Next Generation Sequencing

Third and Fourth Generation Technologies

Why take this course?

πŸŽ‰ Unlock the Secrets of DNA with Next Generation Sequencing (NGS)!

πŸš€ Course Title: Next Generation Sequencing (NGS)

πŸ‘©β€πŸ« Instructor: Anum Ahmad, PhD


Course Headline: Master the Art of Genomic Analysis with NGS πŸ§¬πŸ”¬


Welcome to the World of Next Generation Sequencing (NGS)! Over the past five decades, the field of genomics has been revolutionized by advancements in DNA sequencing technology. The completion of the Human Genome Project marked a turning point, propelling us into a new era of biological discovery and understanding. Today, NGS stands at the forefront of this scientific renaissance, offering unparalleled insights into the genetic blueprints that govern life itself.

Course Description:

NGS technology is a game-changer in the realm of genomic research, transforming the way we analyze DNA and RNA sequences. Unlike the traditional Sanger sequencing method, NGS technologies can process millions of reactions simultaneously, delivering high-throughput sequencing with remarkable speed and efficiency. This course will guide you through the intricacies of NGS, from sample preparation to data analysis, providing a comprehensive understanding of this cutting-edge technology.


What You'll Learn:

πŸ” Understanding the Basics: Dive into the principles of NGS and its significance in modern biology.

πŸ§ͺ Sample Preparation: Explore the process of DNA fragmentation, end repairing, and adaptor ligation that paves the way for library preparation.

βš—οΈ PCR Amplification Techniques: Learn about emulsion PCR and bridge PCR, two critical methods for amplifying your DNA template.

πŸ–₯️ NGS Platforms: Discover the various NGS platforms available, including 454 Pyrosequencing, SOLiD, Ion Torrent Semiconductor Sequencing, and Reversible Terminator Sequencing (Illumina).

πŸ”‹ Data Analysis: Gain insights into how to handle the vast amounts of data generated by NGS.

🧩 Applications of NGS: Explore the myriad applications of NGS in fields such as outbreak management, personalized medicine, cancer research, and more.


Key Steps of NGS:

  1. Sample Preparation (Pre-processing): Tailored to your target DNA or RNA, this step sets the stage for accurate sequencing.
  2. Library Preparation: Involves DNA fragmentation, end repairing, and adaptor ligation to create a library suitable for sequencing.
  3. Sequencing: The core process where the order of nucleotides is determined using high-throughput technology.
  4. Data Analysis (Post-processing): Interpreting the raw data to gain meaningful insights into genomic sequences.

NGS Read Types:

  • Single End Reads: A single sequence from one end of a DNA fragment.
  • Paired End Reads: Both ends of a DNA fragment are sequenced, providing more context and enabling precise location information in the genome.

NGS has opened up new horizons in genomics, allowing researchers to tackle global challenges and advance personalized medicine. With its applications spanning from outbreak management to the detection of viruses and the surveillance of antimicrobial resistance, NGS is a cornerstone technology in modern research.


Who Should Take This Course?

This course is an invaluable resource for students and researchers in fields such as molecular biology, forensic science, medical laboratory technology, biotechnology, and genetics. Whether you're a beginner or looking to deepen your understanding of NGS, this course will equip you with the knowledge to harness the power of genomic analysis.

πŸŽ“ Embark on your learning journey today and become proficient in the world of genomics with Next Generation Sequencing! 🌟

Enroll now and unlock the potential of NGS to propel your research forward!

Related Topics

4506780
udemy ID
21/01/2022
course created date
02/02/2022
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