CRISPR Cas System: Applications in Gene Editing and Beyond

CRISPR in gene editing, gene regulation, ChIP, live-cell imaging, diagnosis etc. | Genetics | Immunology | Biotechnology

4.48 (311 reviews)
Udemy
platform
English
language
Science
category
CRISPR Cas System: Applications in Gene Editing and Beyond
1 564
students
6 hours
content
Mar 2023
last update
$74.99
regular price

What you will learn

Introductory overview of basic concepts of Molecular Biology

What is gene editing?

Gene editing tools: Zinc Finger Nucleases (ZFNs) and TALENs and their limitations

DNA repair by Homology Directed Repair (HDR) pathway and Non Homologous End joining (NHEJ) repair

Introduction to CRISPR-Cas system and how it can be used as a gene editing tool?

Components, types and mechanism of CRISPR-Cas system

Protospacer Adjacent Motifs or PAMs

Off target activity of SpCas9 nuclease

Variants of Cas9 nuclease: Cas9 nickase, dead Cas9 (dCas9) and FokI fused catalytically inactive Cas9,

Cas9 nucleases with novel PAM specificities

High Fidelity SpCas9

Cas9 orthologs: SaCas9, NmCas9, CjCas9

Viral vectors for CRISPR delivery: Retroviral vectors, Adenoviral vectors, AAV vectors, etc.

Prime editing: its components, mechanism, applications, advantages and limitations

CRISPR-mediated Base editing: its components, mechanism, applications, advantages and limitations

CRISPR activation or CRISPRa

CRISPR interference or CRISPRi

Epigenetic editing with dCas9

CRISPR-mediated Chromatin Immunoprecipitation (ChIP)

Fluorescence In Situ Hybridization (FISH)

CRISPR-mediated live-cell imaging of genomic loci

Cas12 and Cas13 enzymes for diagnosing infectious diseases

Sherlock CRISPR-Cas13-based diagnostic kit

DETECTOR: CRISPR-Cas12-based diagnostic kit

FELUDA: CRISPR-Cas19-based diagnostic kit

Why take this course?

🧬 CRISPR Cas System: Applications in Gene Editing and Beyond 🚀

Course Overview

Gene editing with the CRISPR-Cas system has been heralded as one of the biggest technologies of the decade, revolutionizing fields from medicine to agriculture. In this comprehensive course, we'll explore the intricacies of gene editing and its profound impact on various industries. Led by the expert guidance of Dr. Roohi Bansal, you'll gain an advanced understanding of CRISPR's mechanisms, its edge over other gene-editing tools, and a multitude of applications that extend beyond mere genetic manipulation.

Key Learning Points

  • Understanding CRISPR-Cas Functionality: Dive into the science behind how CRISPR functions as a powerful gene-editing tool.

  • Comparison with Other Editing Tools: Learn about CRISPR's place in the gene editing landscape, including ZFNs, TALENs, and more.

  • Components of CRISPR-Cas System: Discover the various elements that make up this versatile system.

  • Beyond Gene Editing: Explore the frontiers where CRISPR is being used to regulate gene expression, edit the epigenome, and offer new treatment options.

  • Live-Cell Imaging and Genome Architecture: Understand how fluorescent proteins are attached to CRISPR components to visualize DNA sequences, chromosomal abnormalities, and the 3D genome architecture.

  • ChIP Enhancements: Learn how CRISPR technology enhances chromatin immunoprecipitation for identifying protein-genomic interactions.

  • Diagnosis and Detection: Discover the role of CRISPR in diagnosing infectious diseases, including the detection of COVID-19.

Course Highlights

🔬 Precise Gene Editing Techniques: Get to grips with advanced techniques like prime editing and base editing, which promise to treat genetic diseases with precision while mitigating potential negative effects.

⚖️ Engineering Cas9 Variants: Explore the engineering of various Cas9 variants and their significance in both gene editing and non-gene editing applications.

📈 Challenges and Limitations: Address the challenges and limitations of the CRISPR-Cas system and discuss ways to overcome them.

Who Should Take This Course?

This course is a treasure trove for anyone with an interest in:

  • Medicine
  • Biotechnology
  • Genetics
  • Immunology
  • Molecular Biology
  • Cell Biology
  • Bioinformatics

Medical students, doctors, and students of biology and its allied disciplines will find this course particularly enlightening. The most complex concepts are made accessible through engaging animations and graphics.

Course Format

The course is designed to be highly interactive and user-friendly, featuring:

  • Detailed video lectures
  • Real-world case studies
  • Interactive quizzes
  • Discussion forums

Why Take This Course?

  • Stay Ahead of the Curve: Get informed about the latest advancements in gene editing and their applications.

  • Expand Your Knowledge Base: Understand the broader implications of CRISPR technology across various fields, from healthcare to agriculture.

  • Expert Insights: Learn from a renowned expert with hands-on experience in CRISPR research.

  • Invaluable Resource: Whether you're a student, researcher, or industry professional, this course will provide the knowledge and skills necessary to navigate and innovate within the realm of gene editing.

Join us on this journey to unravel the mysteries of the CRISPR Cas system and harness its full potential for a better future! 🌟

Screenshots

CRISPR Cas System: Applications in Gene Editing and Beyond - Screenshot_01CRISPR Cas System: Applications in Gene Editing and Beyond - Screenshot_02CRISPR Cas System: Applications in Gene Editing and Beyond - Screenshot_03CRISPR Cas System: Applications in Gene Editing and Beyond - Screenshot_04

Our review

🌟 Overall Course Review 🌟

The course in question receives a high overall rating of 4.55 stars, with recent reviews painting a picture of a comprehensive and engaging learning experience. The course is commended for its structured and categorized approach to teaching concepts, starting from the basics and building up progressively.

Pros:

  • Structured Content: The course is well-organized, with a thoughtful development of concepts from the ground up. This makes it easier for learners to follow along and build their understanding incrementally.

  • Educational Animations: The use of amazing animations adds an element of interest and helps in visualizing complex ideas, enhancing the learning experience.

  • Instructor's Expertise: The instructor is acknowledged as knowledgeable in the field, indicating a high level of expertise and credibility.

  • Detailed Content: The material is explained in detail, providing learners with a thorough understanding of the subject matter.

  • Support for Biotechnology Students: The course content is appreciated by students of biotechnology as it covers fundamental concepts in an accessible manner.

Cons:

  • Accent Challenges: Some learners have difficulty understanding the instructor due to a thick accent, which can be a barrier to comprehension. This issue is subjective and may vary from learner to learner, depending on their individual experiences with diverse accents.

  • Lecture Style Concerns: At least one student found the lectures unsatisfying because the instructor seemed to merely read bullet points without adding personal insights or explanations. This lack of engagement or additional commentary may not provide as rich an experience as some learners expect.

  • Pace of Delivery: A few learners have mentioned that the instructor speaks too quickly, which can make it challenging for those with less prior knowledge of the subject or for non-native English speakers to grasp the content fully.

In summary, this course is highly recommended for its comprehensive and detailed approach to teaching, with a focus on foundational concepts in an engaging manner through animations. However, learners should be aware that there may be challenges related to the instructor's accent and pace of delivery, which could potentially affect the learning experience for some individuals. It's also worth noting that the course content is rich enough to help strengthen the foundation of knowledge for students revising basic concepts. Additionally, there is a mention of an opportunity to store information for future reference, which suggests potential interactive elements within the course platform.

3889916
udemy ID
04/03/2021
course created date
24/08/2022
course indexed date
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