Biotechnology: Basics of gene cloning and gene therapy
Learn about gene cloning and its application in curing inherited diseases and cancer.

What you will learn
Background of gene cloning
What is gene cloning?
Steps involved in gene cloning
What is restriction endonuclease?
Types of restriction endonucleases
Sticky and blunt end restriction
Separation of restricted gene
What is DNA ligase?
Ligation of sticky end and blunt end gene
Differences between restriction enzymes and DNA ligase
What is cloning vector?
Characteristics of cloning vector
Types of cloning vectors
Applications of gene cloning
What is gene therapy?
Gene therapy for inherited diseases
Gene therapy and cancer
Ethical issues raised by gene therapy
Why take this course?
🧬 Mastering Biotechnology: Gene Cloning and Gene Therapy with Dr. Anum Ahmad 🌱
Course Overview:
Discover the foundational principles of biotechnology through the fascinating world of gene cloning and its transformative applications in curing inherited diseases and combating cancer. In this course, you'll unravel the mysteries of DNA manipulation and understand how gene therapy is revolutionizing medicine.
What You'll Learn:
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The Basics of Gene Cloning: Understand how scientists isolate, copy, and insert specific genes into foreign cells to create recombinant DNA molecules.
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Restriction Endonucleases and DNA Ligase: Explore the crucial enzymes that enable the precise cutting and pasting of DNA strands, essential for gene cloning.
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Cloning Vectors: Learn about the various types of vectors used in cloning, and how to select the most appropriate one based on your cloning goals and the size of the insert.
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Gene Therapy: Delve into how gene therapy is employed to treat inherited diseases and cancers by correcting genetic mutations or replacing missing genes.
Key Concepts Covered:
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The Mechanism of Gene Cloning (🔬): A step-by-step guide on how genes are cloned, from isolation to amplification.
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DNA Manipulation Techniques: Master the techniques used for cutting and joining DNA segments, including the roles of restriction endonucleases and DNA ligase.
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Cloning Vectors Explained (🧬➡️🌱): Understand the different types of vectors, their applications, and how to choose the right one for your cloning needs.
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Applications of Gene Cloning: Learn how gene cloning is used in various fields, including medicine, agriculture, and industry.
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Gene Therapy Breakthroughs (🏥✨): Examine the potential of gene therapy to cure diseases by correcting genetic defects or targeting cancerous cells.
Why Take This Course?
This comprehensive course is perfect for:
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Students and Researchers in biology, forensic science, molecular biology, biotechnology, biochemistry, and genetics.
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Those Interested in Advancements in Medicine: Gain insights into how gene cloning and therapy are paving the way for new treatments and therapies.
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Aspiring Biotech Professionals: Acquire the knowledge necessary to enter the booming field of biotechnology with a strong foundation in gene manipulation techniques.
Course Format:
This self-paced online course features engaging video lectures, interactive quizzes, and practical exercises designed to enhance your understanding and application of gene cloning and therapy principles.
Join us on this intellectual expedition to explore the cutting-edge techniques in biotechnology that hold the potential to heal and transform lives. 🌟 Enroll now and be a part of the scientific revolution!