All kinds of plant tissue culture concepts

Who is the father of tissue culture?

Gottlieb Haberlandt

Gottlieb Haberlandt is known as the father of plant tissue culture.

Gottlieb Haberlandt (28 November 1854  to 30 January 1945) was an Austrian botanist. 

His original idea presented in 1902 was called Totipotentiality.

Explant

An explant is the part of a plant which has got the regeneration potential and is capable to give rise to the whole plant.

Step 1  Explant for callus culture

Step 2  Surface sterilization

Step 3  Factors affecting callus culture

          Physical factors

          Growth regulators

Step 4  Suspension culture from callus

Callus culture callus is the undifferentiated and unorganized mass of plant cells

It is basically a tumor tissue which usually forms on wounds of differentiated tissues or organs

Callus formation in vivo is frequently observed as a result of wounds at edges of stems or roots

Explant for callus culture

The explant for callus culture may be the differentiated tissue from any part of the plant

The explant for callus culture may be the differentiated tissue from any part of the plant

The selected explant tissues may be at different stages of cell division cell proliferation and organization  into different distinct specialized structures

If the explant used possesses meristematic cells then the cell division and multiplication will be rapid

Factors affecting callus culture

Many factors are known to influence callus formation in invitro cultures

MS medium most commonly used physical factors  and growth factors

Rest will continue with live session.

Major types of Media

MS media

B5 media

N6 Media

Niches media

Classification of Plant tissue culture

inorganic nutrients

Carbon Energy sources

Macro nutrients

Micro nutrients

Difference between macro and micro nutrients

Organic supplements

Vitamins

Amino acids

other organic supplements

activated charcoal

Antibiotics

Growth regulators

Auxins

Cytokinin

Gibberellins

Plant growth promoters

Plant growth functions

Plant growth applications

commercial usage

plant growth inhibitors

ABA

applications of ABA

effects of ABA

Ethaline uses

Solidifying agents

Gelatin

PH of Medium

Suitable, selection and sterilization of medium

Overview